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Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV220389	1/2	Homo sapiens	Blood plasma	ExoQuick	Murugesan S	2022	63%
Study summary Full title Extracellular Vesicles From Women With Severe Preeclampsia Impair Vascular Endothelial Function. All authors Murugesan S, Hussey H, Saravanakumar L, Sinkey RG, Sturdivant AB, Powell MF, Berkowitz DE Journal Anesth Analg Abstract Preeclampsia (PE) manifesting as hypertension and organ injury is mediated by vascular dysfunction. (show more...)Preeclampsia (PE) manifesting as hypertension and organ injury is mediated by vascular dysfunction. In biological fluids, extracellular vesicles (EVs) containing microRNA (miRNA), protein, and other cargo released from the placenta may serve as carriers to propagate injury, altering the functional phenotype of endothelial cells. PE has been consistently correlated with increased levels of placenta-derived EVs (pEVs) in maternal circulation. However, whether pEVs impaired endothelial cell function remains to be determined. In this study, we hypothesize that pEVs from pregnant women with severe PE (sPE) impair endothelial function through altered cell signaling. (hide) EV-METRIC 63% (91st percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Blood plasma Sample origin Healthy pregnant Focus vesicles extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture ExoQuick Protein markers EV: CD63/ Flotillin1/ PLAP/ TSG101 non-EV: Apolipoprotein A1/ GM130 Proteomics no Show all info Study aim Function Sample Species Homo sapiens Sample Type Blood plasma Separation Method Commercial kit ExoQuick Other Name other separation method ExoQuick Characterization: Protein analysis Protein Concentration Method BCA Protein Yield (µg) per milliliter of starting sample Western Blot Antibody details provided? Yes Antibody dilution provided? Yes Lysis buffer provided? Yes Detected EV-associated proteins CD63/ Flotillin1 Not detected contaminants Apolipoprotein A1/ GM130 ELISA Antibody details provided? Yes Antibody dilution provided? Yes Detected EV-associated proteins CD63/ PLAP Other 1 Confocal microscopy Detected EV-associated proteins TSG101 Characterization: Lipid analysis No Characterization: Particle analysis NTA Report type Mean Reported size (nm) 101.3 EV concentration Yes Particle yield particles per milliliter of starting sample: 1.28E+06 EM EM-type Transmission-EM Image type Close-up Report size (nm) 30-150

	EV220389	2/2	Homo sapiens	Blood plasma	ExoQuick	Murugesan S	2022	63%
Study summary Full title Extracellular Vesicles From Women With Severe Preeclampsia Impair Vascular Endothelial Function. All authors Murugesan S, Hussey H, Saravanakumar L, Sinkey RG, Sturdivant AB, Powell MF, Berkowitz DE Journal Anesth Analg Abstract Preeclampsia (PE) manifesting as hypertension and organ injury is mediated by vascular dysfunction. (show more...)Preeclampsia (PE) manifesting as hypertension and organ injury is mediated by vascular dysfunction. In biological fluids, extracellular vesicles (EVs) containing microRNA (miRNA), protein, and other cargo released from the placenta may serve as carriers to propagate injury, altering the functional phenotype of endothelial cells. PE has been consistently correlated with increased levels of placenta-derived EVs (pEVs) in maternal circulation. However, whether pEVs impaired endothelial cell function remains to be determined. In this study, we hypothesize that pEVs from pregnant women with severe PE (sPE) impair endothelial function through altered cell signaling. (hide) EV-METRIC 63% (91st percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Blood plasma Sample origin Severe pre-eclampsia Focus vesicles extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture ExoQuick Protein markers EV: CD63/ Flotillin1/ PLAP/ TSG101 non-EV: Apolipoprotein A1/ GM130 Proteomics no Show all info Study aim Function Sample Species Homo sapiens Sample Type Blood plasma Separation Method Commercial kit ExoQuick Other Name other separation method ExoQuick Characterization: Protein analysis Protein Concentration Method BCA Protein Yield (µg) per milliliter of starting sample Western Blot Antibody details provided? Yes Antibody dilution provided? Yes Lysis buffer provided? Yes Detected EV-associated proteins CD63/ Flotillin1 Not detected contaminants Apolipoprotein A1/ GM130 ELISA Antibody details provided? Yes Antibody dilution provided? Yes Detected EV-associated proteins CD63/ PLAP Other 1 Confocal microscopy Detected EV-associated proteins TSG101 Characterization: Lipid analysis No Characterization: Particle analysis NTA Report type Mean Reported size (nm) 108.8 EV concentration Yes Particle yield particles per milliliter of starting sample: 1.63E+06 EM EM-type Transmission-EM Image type Close-up Report size (nm) 30-150

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EV-TRACK ID	EV220389
species	Homo sapiens
sample type	Blood plasma
condition	Healthy pregnant	Severe pre-eclampsia
separation protocol	ExoQuick	ExoQuick
Exp. nr.	1	2
EV-METRIC %	63	63