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You searched for: EV210338 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210338 | 1/1 | Bos taurus | 1% skim milk | (d)(U)C | Fang, Zhou | 2022 | 56% | |
Study summaryFull title
All authors
Fang Zhou, Pearl Ebea, Ezra Mutai, Haichuan Wang, Sonal Sukreet, Shya Navazesh, Haluk Dogan, Wenhao Li, Juan Cui, Peng Ji, Denise M O Ramirez, Janos Zempleni
Journal
Frontiers Nutr.
Abstract
Human milk contains large amounts of small extracellular vesicles (sEVs) and their microRNA cargos, (show more...)
EV-METRIC
56% (16th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
1% skim milk
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ CD9/ CD63/ CD81/ Integrin-beta/ Histone H3/ Apolipoprotein B
non-EV: None Proteomics
no
Show all info
Study aim
Function/Mechanism of uptake/transfer
Sample
Species
Bos taurus
Sample Type
1% skim milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
Fiberlite-F37L-8x100 rotor
Pelleting: speed (g)
120,000
Wash: volume per pellet (ml)
50
Wash: time (min)
90
Wash: Rotor Type
Fiberlite-F37-8x-100 rotor
Wash: speed (g)
120,000
Characterization: Protein analysis
Protein Concentration Method
BCA/ Qubit
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Alix/ CD9/ CD63/ CD81
Not detected EV-associated proteins
Integrin-beta/ Histone H3/ Apolipoprotein B
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
106.6 +/- 6.7
EV concentration
Yes
EM
EM-type
Scanning-EM
Image type
Wide-field
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1 - 1 of 1 |
EV-TRACK ID | EV210338 |
---|---|
species | Bos taurus |
sample type | 1% skim milk |
condition | Control condition |
separation protocol | dUC |
Exp. nr. | 1 |
EV-METRIC % | 56 |