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You searched for: EV200118 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV200118 | 1/1 | Homo sapiens | primary bone marrow stromal cells |
(d)(U)C ExoQuick Filtration UF |
Tu, Chenggong | 2021 | 75% | |
Study summaryFull title
All authors
Chenggong Tu, Zhimin Du, Hui Zhang, Yueyuan Feng, Yujun Qi, Yongjiang Zheng, Jinbao Liu, Jinheng Wang
Journal
Theranostics
Abstract
Extracellular vesicles (EVs), including exosomes and microvesicles, derived from bone marrow stromal (show more...)
EV-METRIC
75% (96th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Commercial method Filtration Ultrafiltration Protein markers
EV: CD63/ Flotillin1/ CD9
non-EV: Calreticulin Proteomics
no
Show all info
Study aim
Mechanism of uptake/transfer
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
primary bone marrow stromal cells
EV-harvesting Medium
Serum free medium
Cell viability (%)
95
Cell count
2000000/flask
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Pelleting performed
No
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Regenerated cellulose
Commercial kit
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
Flotillin1/ CD9/ CD63
Not detected contaminants
Calreticulin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
30-210
EV concentration
Yes
Particle yield
Yes, as number of particles per milliliter of starting sample 9.00E+07
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
50-120
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1 - 1 of 1 |
EV-TRACK ID | EV200118 |
---|---|
species | Homo sapiens |
sample type | Cell culture |
cell type | primary bone marrow stromal cells |
condition | Control condition |
separation protocol | dUC ExoQuick Filtration Ultrafiltration |
Exp. nr. | 1 |
EV-METRIC % | 75 |