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Details	EV-TRACK ID	Experiment nr.	Species	Sample type	Separation protocol	First author	Year	EV-METRIC
	EV220329	1/2	Homo sapiens	Blood plasma	(d)(U)C	Chong MC	2024	56%
Study summary Full title Acute exercise-induced release of innate immune proteins via small extracellular vesicles changes with aerobic fitness and age. All authors Chong MC, Shah AD, Schittenhelm RB, Silva A, James PF, Wu SSX, Howitt J Journal Acta Physiol (Oxf) Abstract Physical exercise triggers the secretion of small extracellular vesicles (sEVs) into the circulation (show more...)Physical exercise triggers the secretion of small extracellular vesicles (sEVs) into the circulation in humans, enabling signalling crosstalk between tissues. Exercise-derived EVs and their cargo have been proposed to mediate adaptations to exercise/ however, our understanding of how exercise-derived EV protein cargo is modulated by factors such as aerobic fitness and age of an individual is currently unknown. Here, we examined the circulating sEV proteome following aerobic exercise in healthy males of different ages and aerobic fitness to understand exercise-induced EV response during the aging process. (hide) EV-METRIC 56% (88th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Blood plasma Sample origin Control condition Focus vesicles extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (Differential) (ultra)centrifugation Protein markers EV: TSG101/ Syntenin-1 non-EV: ApoA-1/ GM130 Proteomics yes Show all info Study aim Identification of content (omics approaches) Sample Species Homo sapiens Sample Type Blood plasma Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed Yes Pelleting: rotor type Type 70 Ti Pelleting: speed (g) 100,000 Wash: volume per pellet (ml) 20 Wash: time (min) 60 Wash: Rotor Type Type 70 Ti Wash: speed (g) 100,000 Characterization: Protein analysis Protein Concentration Method microBCA Western Blot Antibody details provided? No Detected EV-associated proteins TSG101/ Syntenin-1 Detected contaminants ApoA-1 Not detected contaminants GM130 Proteomics database No Characterization: Lipid analysis No Other particle analysis name(1) Microfluidic resistive pulse sensing Report type Mean Report size 88 EV-concentration Yes Particle yield as number of particles per milliliter of starting sample: 2.80E+10

	EV220329	2/2	Homo sapiens	Blood plasma	(d)(U)C	Chong MC	2024	56%
Study summary Full title Acute exercise-induced release of innate immune proteins via small extracellular vesicles changes with aerobic fitness and age. All authors Chong MC, Shah AD, Schittenhelm RB, Silva A, James PF, Wu SSX, Howitt J Journal Acta Physiol (Oxf) Abstract Physical exercise triggers the secretion of small extracellular vesicles (sEVs) into the circulation (show more...)Physical exercise triggers the secretion of small extracellular vesicles (sEVs) into the circulation in humans, enabling signalling crosstalk between tissues. Exercise-derived EVs and their cargo have been proposed to mediate adaptations to exercise/ however, our understanding of how exercise-derived EV protein cargo is modulated by factors such as aerobic fitness and age of an individual is currently unknown. Here, we examined the circulating sEV proteome following aerobic exercise in healthy males of different ages and aerobic fitness to understand exercise-induced EV response during the aging process. (hide) EV-METRIC 56% (88th percentile of all experiments on the same sample type) Reported Not reported Not applicable EV-enriched proteins Protein analysis: analysis of three or more EV-enriched proteins non EV-enriched protein Protein analysis: assessment of a non-EV-enriched protein qualitative and quantitative analysis Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected. electron microscopy images Particle analysis: inclusion of a widefield and close-up electron microscopy image density gradient Separation method: density gradient, at least as validation of results attributed to EVs EV density Separation method: reporting of obtained EV density ultracentrifugation specifics Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps antibody specifics Protein analysis: antibody clone/reference number and dilution lysate preparation Protein analysis: lysis buffer composition Study data Sample type Blood plasma Sample origin post-exercise Focus vesicles extracellular vesicle Separation protocol Separation protocol Gives a short, non-chronological overview of the different steps of the separation protocol. dUC = (Differential) (ultra)centrifugation DG = density gradient UF = ultrafiltration SEC = size-exclusion chromatography IAF = immuno-affinity capture (Differential) (ultra)centrifugation Protein markers EV: TSG101/ Syntenin-1 non-EV: ApoA-1/ GM130 Proteomics yes Show all info Study aim Identification of content (omics approaches) Sample Species Homo sapiens Sample Type Blood plasma Separation Method (Differential) (ultra)centrifugation dUC: centrifugation steps Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed Yes Pelleting: rotor type Type 70 Ti Pelleting: speed (g) 100,000 Wash: volume per pellet (ml) 20 Wash: time (min) 60 Wash: Rotor Type Type 70 Ti Wash: speed (g) 100,000 Characterization: Protein analysis Protein Concentration Method microBCA Western Blot Antibody details provided? No Detected EV-associated proteins TSG101/ Syntenin-1 Detected contaminants ApoA-1 Not detected contaminants GM130 Proteomics database No Characterization: Lipid analysis No Other particle analysis name(1) Microfluidic resistive pulse sensing Report type Mean Report size 89 EV-concentration Yes Particle yield as number of particles per milliliter of starting sample: 3.90E+10

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EV-TRACK ID	EV220329
species	Homo sapiens
sample type	Blood plasma
condition	Control condition	post-exercise
separation protocol	dUC	dUC
Exp. nr.	1	2
EV-METRIC %	56	56