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You searched for: EV220058 (EV-TRACK ID)
Showing 1 - 5 of 5
Showing 1 - 5 of 5
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV220058 | 1/5 | Homo sapiens | HCT-116 | (d)(U)C | Lee CH | 2018 | 44% | |
Study summaryFull title
All authors
Lee CH, Im EJ, Moon PG, Baek MC
Journal
BMC Cancer
Abstract
Small extracellular vesicles (small-EVs) are membranous vesicles that contain unique information reg (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ HSP70/ TSPAN1/ LGALS3BP/ SLC1A/ CLDN7/ GPRC5A/ CD63
non-EV: None Proteomics
yes
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HCT-116
EV-harvesting Medium
EV-depleted medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000
Wash: time (min)
70
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ TSPAN1/ LGALS3BP/ SLC1A/ CLDN7/ GPRC5A/ HSP70/ Alix
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
1-500
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
|
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EV220058 | 2/5 | Homo sapiens | HT-29 | (d)(U)C | Lee CH | 2018 | 44% | |
Study summaryFull title
All authors
Lee CH, Im EJ, Moon PG, Baek MC
Journal
BMC Cancer
Abstract
Small extracellular vesicles (small-EVs) are membranous vesicles that contain unique information reg (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: Alix/ HSP70/ TSPAN1/ LGALS3BP/ SLC1A/ CLDN7/ GPRC5A/ CD63
non-EV: None Proteomics
yes
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HT-29
EV-harvesting Medium
EV-depleted medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000
Wash: time (min)
70
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD63/ TSPAN1/ LGALS3BP/ SLC1A/ CLDN7/ GPRC5A/ HSP70/ Alix
Proteomics database
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
1-500
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Wide-field
|
||||||||
EV220058 | 4/5 | Homo sapiens | Blood plasma | ExoQuick | Lee CH | 2018 | 38% | |
Study summaryFull title
All authors
Lee CH, Im EJ, Moon PG, Baek MC
Journal
BMC Cancer
Abstract
Small extracellular vesicles (small-EVs) are membranous vesicles that contain unique information reg (show more...)
EV-METRIC
38% (72nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Commercial method
Protein markers
EV: TSPAN1/ CD63/ CD9/ TSPAN1/ LGALS3BP/ SLC1A5/ CLDN7/ GPRC5A
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Commercial kit
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
TSPAN1/ LGALS3BP/ SLC1A5/ CLDN7/ GPRC5A/ CD9
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD63/ TSPAN1
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220058 | 5/5 | Homo sapiens | Blood plasma | ExoQuick | Lee CH | 2018 | 38% | |
Study summaryFull title
All authors
Lee CH, Im EJ, Moon PG, Baek MC
Journal
BMC Cancer
Abstract
Small extracellular vesicles (small-EVs) are membranous vesicles that contain unique information reg (show more...)
EV-METRIC
38% (72nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Colon cancer
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Commercial method
Protein markers
EV: TSPAN1/ CD63/ CD9/ TSPAN1/ LGALS3BP/ SLC1A5/ CLDN7/ GPRC5A
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Commercial kit
ExoQuick
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
TSPAN1/ LGALS3BP/ SLC1A5/ CLDN7/ GPRC5A/ CD9
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD63/ TSPAN1
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV220058 | 3/5 | Homo sapiens | CRL-1541 | (d)(U)C | Lee CH | 2018 | 33% | |
Study summaryFull title
All authors
Lee CH, Im EJ, Moon PG, Baek MC
Journal
BMC Cancer
Abstract
Small extracellular vesicles (small-EVs) are membranous vesicles that contain unique information reg (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: CLDN7/ GPRC5A/ HSP70/ TSPAN1/ LGALS3BP/ SLC1A5
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
CRL-1541
EV-harvesting Medium
EV-depleted medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
100000
Wash: time (min)
70
Wash: speed (g)
100000
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
HSP70/ CLDN7/ GPRC5A
Not detected EV-associated proteins
TSPAN1/ LGALS3BP/ SLC1A5
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
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1 - 5 of 5 |
EV-TRACK ID | EV220058 | ||||
---|---|---|---|---|---|
species | Homo sapiens | ||||
sample type | Cell culture | Cell culture | Blood plasma | Blood plasma | Cell culture |
cell type | HCT-116 | HT-29 | NA | NA | CRL-1541 |
medium | EV-depleted medium | EV-depleted medium | NA | NA | EV-depleted medium |
condition | Control condition | Control condition | Control condition | Colon cancer | Control condition |
separation protocol | dUC | dUC | ExoQuick | ExoQuick | dUC |
Exp. nr. | 1 | 2 | 4 | 5 | 3 |
EV-METRIC % | 44 | 44 | 38 | 38 | 33 |