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You searched for: EV210378 (EV-TRACK ID)
Showing 1 - 10 of 10
Showing 1 - 10 of 10
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV210378 | 9/10 | Homo sapiens | MCF-7 |
(d)(U)C Filtration |
Jin D | 2018 | 44% | |
Study summaryFull title
All authors
Jin D, Yang F, Zhang Y, Liu L, Zhou Y, Wang F, Zhang GJ
Journal
Anal Chem
Abstract
Tumor exosomes that inherit molecular markers from their parent cells are emerging as cellular "surr (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD63/ EpCAM/ CEA/ PTK7/ AFP/ PSMA/ PDGF
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MCF-7
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
110000
Wash: time (min)
70
Wash: speed (g)
110000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63
Detected EV-associated proteins
CD63/ EpCAM/ CEA/ PTK7/ AFP/ PSMA/ PDGF
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
126
EV concentration
Yes
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
90
|
||||||||
EV210378 | 7/10 | Homo sapiens | MCF10A |
(d)(U)C Filtration |
Jin D | 2018 | 22% | |
Study summaryFull title
All authors
Jin D, Yang F, Zhang Y, Liu L, Zhou Y, Wang F, Zhang GJ
Journal
Anal Chem
Abstract
Tumor exosomes that inherit molecular markers from their parent cells are emerging as cellular "surr (show more...)
EV-METRIC
22% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD63/ EpCAM/ CEA/ PTK7/ AFP/ PSMA/ PDGF
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MCF10A
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
110000
Wash: time (min)
70
Wash: speed (g)
110000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Antibody dilution provided?
Yes
Lysis buffer provided?
Yes
Detected EV-associated proteins
CD9/ CD63
Detected EV-associated proteins
CD63/ EpCAM/ CEA/ PTK7/ AFP/ PSMA/ PDGF
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210378 | 2/10 | Homo sapiens | HepG2 |
(d)(U)C Filtration |
Jin D | 2018 | 14% | |
Study summaryFull title
All authors
Jin D, Yang F, Zhang Y, Liu L, Zhou Y, Wang F, Zhang GJ
Journal
Anal Chem
Abstract
Tumor exosomes that inherit molecular markers from their parent cells are emerging as cellular "surr (show more...)
EV-METRIC
14% (44th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD63/ EpCAM/ CEA/ PTK7/ AFP/ PSMA/ PDGF
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HepG2
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
110000
Wash: time (min)
70
Wash: speed (g)
110000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Detected EV-associated proteins
CD63/ EpCAM/ CEA/ PTK7/ AFP/ PSMA/ PDGF
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210378 | 3/10 | Homo sapiens | SGC7901 |
(d)(U)C Filtration |
Jin D | 2018 | 14% | |
Study summaryFull title
All authors
Jin D, Yang F, Zhang Y, Liu L, Zhou Y, Wang F, Zhang GJ
Journal
Anal Chem
Abstract
Tumor exosomes that inherit molecular markers from their parent cells are emerging as cellular "surr (show more...)
EV-METRIC
14% (44th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD63/ EpCAM/ CEA/ PTK7/ AFP/ PSMA/ PDGF
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
SGC7901
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
110000
Wash: time (min)
70
Wash: speed (g)
110000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Detected EV-associated proteins
CD63/ EpCAM/ CEA/ PTK7/ AFP/ PSMA/ PDGF
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210378 | 5/10 | Homo sapiens | HeLa |
(d)(U)C Filtration |
Jin D | 2018 | 14% | |
Study summaryFull title
All authors
Jin D, Yang F, Zhang Y, Liu L, Zhou Y, Wang F, Zhang GJ
Journal
Anal Chem
Abstract
Tumor exosomes that inherit molecular markers from their parent cells are emerging as cellular "surr (show more...)
EV-METRIC
14% (44th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD63/ EpCAM/ CEA/ PTK7/ AFP/ PSMA/ PDGF
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HeLa
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: speed (g)
110000
Wash: time (min)
70
Wash: speed (g)
110000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Detected EV-associated proteins
CD63/ EpCAM/ CEA/ PTK7/ AFP/ PSMA/ PDGF
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210378 | 1/10 | Homo sapiens | HepG2 |
(d)(U)C Filtration |
Jin D | 2018 | 0% | |
Study summaryFull title
All authors
Jin D, Yang F, Zhang Y, Liu L, Zhou Y, Wang F, Zhang GJ
Journal
Anal Chem
Abstract
Tumor exosomes that inherit molecular markers from their parent cells are emerging as cellular "surr (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: EpCAM/ CD63
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HepG2
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: speed (g)
20000
Filtration steps
> 0.45 µm,
Characterization: Protein analysis
Protein Concentration Method
BCA
Detected EV-associated proteins
CD63/ EpCAM
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210378 | 4/10 | Homo sapiens | SGC7901 |
(d)(U)C Filtration |
Jin D | 2018 | 0% | |
Study summaryFull title
All authors
Jin D, Yang F, Zhang Y, Liu L, Zhou Y, Wang F, Zhang GJ
Journal
Anal Chem
Abstract
Tumor exosomes that inherit molecular markers from their parent cells are emerging as cellular "surr (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
SGC7901
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: speed (g)
20000
Filtration steps
> 0.45 µm,
Characterization: Protein analysis
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210378 | 6/10 | Homo sapiens | HeLa |
(d)(U)C Filtration |
Jin D | 2018 | 0% | |
Study summaryFull title
All authors
Jin D, Yang F, Zhang Y, Liu L, Zhou Y, Wang F, Zhang GJ
Journal
Anal Chem
Abstract
Tumor exosomes that inherit molecular markers from their parent cells are emerging as cellular "surr (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HeLa
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: speed (g)
20000
Filtration steps
> 0.45 µm,
Characterization: Protein analysis
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210378 | 8/10 | Homo sapiens | MCF10A |
(d)(U)C Filtration |
Jin D | 2018 | 0% | |
Study summaryFull title
All authors
Jin D, Yang F, Zhang Y, Liu L, Zhou Y, Wang F, Zhang GJ
Journal
Anal Chem
Abstract
Tumor exosomes that inherit molecular markers from their parent cells are emerging as cellular "surr (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MCF10A
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: speed (g)
20000
Filtration steps
> 0.45 µm,
Characterization: Protein analysis
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV210378 | 10/10 | Homo sapiens | MCF-7 |
(d)(U)C Filtration |
Jin D | 2018 | 0% | |
Study summaryFull title
All authors
Jin D, Yang F, Zhang Y, Liu L, Zhou Y, Wang F, Zhang GJ
Journal
Anal Chem
Abstract
Tumor exosomes that inherit molecular markers from their parent cells are emerging as cellular "surr (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
MCF-7
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: speed (g)
20000
Filtration steps
> 0.45 µm,
Characterization: Protein analysis
Protein Concentration Method
BCA
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
1 - 10 of 10 |
EV-TRACK ID | EV210378 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
species | Homo sapiens | |||||||||
sample type | Cell culture | |||||||||
cell type | MCF-7 | MCF10A | HepG2 | SGC7901 | HeLa | HepG2 | SGC7901 | HeLa | MCF10A | MCF-7 |
condition | Control condition | Control condition | Control condition | Control condition | Control condition | Control condition | Control condition | Control condition | Control condition | Control condition |
separation protocol | dUC/ Filtration | dUC/ Filtration | dUC/ Filtration | dUC/ Filtration | dUC/ Filtration | dUC/ Filtration | dUC/ Filtration | dUC/ Filtration | dUC/ Filtration | dUC/ Filtration |
Exp. nr. | 9 | 7 | 2 | 3 | 5 | 1 | 4 | 6 | 8 | 10 |
EV-METRIC % | 44 | 22 | 14 | 14 | 14 | 0 | 0 | 0 | 0 | 0 |