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You searched for: EV130131 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130131 | 1/1 | Equus caballus | Equine seminal fluid |
(d)(U)C DG Gel filtration |
Aalberts M | 2013 | 38% | |
Study summaryFull title
All authors
Aalberts M, Sostaric E, Wubbolts R, Wauben MW, Nolte-'t Hoen EN, Gadella BM, Stout TA, Stoorvogel W
Journal
Biochim Biophys Acta Proteins Proteom
Abstract
Seminal plasma contains various types of extracellular vesicles, including prostasomes. Prostasomes (show more...)
EV-METRIC
38% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Equine seminal fluid
Sample origin
NAY
Focus vesicles
Prostasomes
Separation protocol
Separation protocol
(d)(U)C
DG Gel filtration Protein markers
EV: CD9
non-EV: Proteomics
no
EV density (g/ml)
1.21
Show all info
Study aim
Function
Sample
Species
Equus caballus
Sample Type
Equine seminal fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Density gradient
Lowest density fraction
0.4
Highest density fraction
2
Orientation
Bottom-up
Rotor type
TLA55
Speed (g)
108000
Other
Name other separation method
Gel filtration
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
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EV-TRACK ID | EV130131 |
---|---|
species | Equus caballus |
sample type | Equine seminal fluid |
condition | NAY |
separation protocol | (d)(U)C DG Gel filtration |
Exp. nr. | 1 |
EV-METRIC % | 38 |