Search > Results
You searched for: EV130005 (EV-TRACK ID)
Showing 1 - 1 of 1
Showing 1 - 1 of 1
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130005 | 1/1 | Homo sapiens | NAY | (d)(U)C | Honegger A | 2013 | 56% | |
Study summaryFull title
All authors
Honegger A, Leitz J, Bulkescher J, Hoppe-Seyler K, Hoppe-Seyler F
Journal
Int J Cancer
Abstract
The human papillomavirus (HPV) E6/E7 oncogenes play a crucial role in the HPV-induced carcinogenesis (show more...)
EV-METRIC
56% (90th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
microvesicles
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
253.9 (pelleting) / 253.9 (washing)
Protein markers
EV: TSG101/ Survivin/ CD63/ Hsc70/ Annexin1/ Beta-actin/ CD9
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW28
Pelleting: adjusted k-factor
253.9
Wash: volume per pellet (ml)
36
Wash: Rotor Type
SW28
Wash: adjusted k-factor
253.9
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD63/ CD9/ TSG101/ Beta-actin/ Annexin1/ Hsc70/ Survivin
Detected contaminants
Cell organelle protein
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Beta-actin/ Annexin1/ Hsc70/ Survivin
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
1 - 1 of 1 |
EV-TRACK ID | EV130005 |
---|---|
species | Homo sapiens |
sample type | Cell culture |
cell type | NAY |
condition | NAY |
separation protocol | (d)(U)C |
Exp. nr. | 1 |
EV-METRIC % | 56 |