Search > Results
You searched for: EV110026 (EV-TRACK ID)
Showing 1 - 4 of 4
Showing 1 - 4 of 4
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV110026 | 2/4 | Homo sapiens | Saliva | (d)(U)C | Berckmans RJ | 2011 | 44% | |
Study summaryFull title
All authors
Berckmans RJ, Sturk A, van Tienen LM, Schaap MC, Nieuwland R
Journal
Blood
Abstract
On vascular damage, coagulation is initiated by extravascular tissue factor (TF). Intravascular TF, (show more...)
EV-METRIC
44% (63rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Saliva
Sample origin
NAY
Focus vesicles
Vesicles
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
472.2 (pelleting)
Protein markers
EV: TF
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Saliva
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Pelleting: rotor type
TLA55
Pelleting: adjusted k-factor
472.2
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TF
ELISA
Antibody details provided?
No
Detected EV-associated proteins
TF
Characterization: Particle analysis
EM
EM-type
immune EM
EM protein
tissue factor; CD63; CD133
Image type
Close-up, Wide-field
|
||||||||
EV110026 | 4/4 | Homo sapiens | Saliva | (d)(U)C | Berckmans RJ | 2011 | 44% | |
Study summaryFull title
All authors
Berckmans RJ, Sturk A, van Tienen LM, Schaap MC, Nieuwland R
Journal
Blood
Abstract
On vascular damage, coagulation is initiated by extravascular tissue factor (TF). Intravascular TF, (show more...)
EV-METRIC
44% (63rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Saliva
Sample origin
NAY
Focus vesicles
Vesicles
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
57.93 (pelleting)
Protein markers
EV: TF
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Saliva
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
TLA55
Pelleting: adjusted k-factor
57.93
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TF
ELISA
Antibody details provided?
No
Detected EV-associated proteins
TF
Characterization: Particle analysis
EM
EM-type
immune EM
EM protein
tissue factor; CD63; CD134
Image type
Close-up, Wide-field
|
||||||||
EV110026 | 1/4 | Homo sapiens | Blood plasma | (d)(U)C | Berckmans RJ | 2011 | 22% | |
Study summaryFull title
All authors
Berckmans RJ, Sturk A, van Tienen LM, Schaap MC, Nieuwland R
Journal
Blood
Abstract
On vascular damage, coagulation is initiated by extravascular tissue factor (TF). Intravascular TF, (show more...)
EV-METRIC
22% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
Vesicles
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
472.2 (pelleting)
Protein markers
EV: TF
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
Yes
Pelleting: time(min)
30
Pelleting: rotor type
TLA55
Pelleting: adjusted k-factor
472.2
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TF
ELISA
Antibody details provided?
No
Detected EV-associated proteins
TF
Characterization: Particle analysis
None
|
||||||||
EV110026 | 3/4 | Homo sapiens | Blood plasma | (d)(U)C | Berckmans RJ | 2011 | 22% | |
Study summaryFull title
All authors
Berckmans RJ, Sturk A, van Tienen LM, Schaap MC, Nieuwland R
Journal
Blood
Abstract
On vascular damage, coagulation is initiated by extravascular tissue factor (TF). Intravascular TF, (show more...)
EV-METRIC
22% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
Vesicles
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
57.93 (pelleting)
Protein markers
EV: TF
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
TLA55
Pelleting: adjusted k-factor
57.93
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TF
ELISA
Antibody details provided?
No
Detected EV-associated proteins
TF
Characterization: Particle analysis
None
|
||||||||
1 - 4 of 4 |
EV-TRACK ID | EV110026 | |||
---|---|---|---|---|
species | Homo sapiens | |||
sample type | Saliva | Saliva | Blood plasma | Blood plasma |
condition | NAY | NAY | NAY | NAY |
separation protocol | (d)(U)C | (d)(U)C | (d)(U)C | (d)(U)C |
Exp. nr. | 2 | 4 | 1 | 3 |
EV-METRIC % | 44 | 44 | 22 | 22 |