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You searched for: EV110022 (EV-TRACK ID)
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Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV110022 | 1/1 | Homo sapiens | Urine |
(d)(U)C DG |
Staubach S | 2011 | 33% | |
Study summaryFull title
All authors
Staubach S, Schadewaldt P, Wendel U, Nohroudi K, Hanisch FG
Journal
J Proteome Res
Abstract
A variety of genetic variations in the galactose-1-phosphate uridyltransferase (GALT) gene cause pro (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
Exovesicles
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Alix/ HSP70/ MUC1/ Flotillin2
non-EV: Tamm-Horsfall glycoprotein Proteomics
yes
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Wash: volume per pellet (ml)
1.5
Density gradient
Only used for validation of main results
Yes
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ HSP70/ Flotillin2/ MUC1
Detected contaminants
Tamm-Horsfall glycoprotein
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Flotillin2/ MUC1
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
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EV-TRACK ID | EV110022 |
---|---|
species | Homo sapiens |
sample type | Urine |
condition | NAY |
separation protocol | (d)(U)C DG |
Exp. nr. | 1 |
EV-METRIC % | 33 |