Search > Results
You searched for: EV110008 (EV-TRACK ID)
Showing 1 - 1 of 1
Showing 1 - 1 of 1
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV110008 | 1/1 | Mus musculus | NAY |
(d)(U)C DG |
Lee YS | 2011 | 33% | |
Study summaryFull title
All authors
Lee YS, Kim SH, Cho JA, Kim CW
Journal
Exp Mol Med
Abstract
Exosomes are small membrane vesicles secreted from various types of cells. Tumor-derived exosomes co (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: TSG101/ MHC2/ Hsc70/ MHC1
non-EV: Tubulin/ Cell organelle protein Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2.5
Orientation
Bottom-up
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
TSG101/ MHC1/ MHC2/ Hsc70
Detected contaminants
Cell organelle protein/ Tubulin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
MHC1/ MHC2/ Hsc70
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
1 - 1 of 1 |
EV-TRACK ID | EV110008 |
---|---|
species | Mus musculus |
sample type | Cell culture |
cell type | NAY |
condition | NAY |
separation protocol | (d)(U)C DG |
Exp. nr. | 1 |
EV-METRIC % | 33 |