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You searched for: 2024 (Year of publication)
Showing 51 - 70 of 70
Showing 51 - 70 of 70
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV240006 | 4/4 | Mus musculus | Neuro-2a (N2a) CCL-131 |
(d)(U)C Total Exosome Isolation lipid-based affinity capture |
Weerakkody, Jonathan S. | 2024 | 50% | |
Study summaryFull title
All authors
Jonathan S. Weerakkody, Tiffany Tseng, Mackenzie Topper, Sikha Thoduvayil, Abhijith Radhakrishnan, Frederic Pincet, Themis R. Kyriakides, Roshan W. Gunasekara, Sathish Ramakrishnan
Journal
Abstract
The biggest challenge in current isolation methods for lipid bilayer-encapsulated vesicles, such as (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small extracellular vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Total Exosome Isolation lipid-based affinity capture Protein markers
EV: CD9/ CD81
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
Neuro-2a (N2a) CCL-131
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: rotor type
SW 55 Ti
Pelleting: speed (g)
12500
Commercial kit
Total Exosome Isolation
Other
Name other separation method
Total Exosome Isolation
Other
Name other separation method
lipid-based affinity capture
EV-subtype
Distinction between multiple subtypes
Size
Used subtypes
150-350
Characterization: Protein analysis
Protein Concentration Method
Not determined
Detected EV-associated proteins
CD9/ CD81
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
105
NTA
Report type
Mean
Reported size (nm)
122
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 3.00E+07
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Other particle analysis name(1)
dSTORM single molecule localization microscopy
Report type
Mean
Report size
52
EV-concentration
No
Extra information
This paper was to validate the efficacy of photosensitive lipid nanoprobe for the isolation and size selective enrichment of native extracellular vesicles
|
||||||||
EV230572 | 2/6 | Homo sapiens | HEK293 | PEI precipitation | Djeungoue-Petga, Marie-Ange | 2024 | 50% | |
Study summaryFull title
All authors
Marie Ange Djeungoue Petgaa, Catherine Taylora, Alexander Macpherson, Surendar Reddy Dhadi, Thomas Rollin, Jeremy W. Roya, Anirban Ghosh, Stephen M. Lewis, Rodney J. Ouellette
Journal
Abstract
Extracellular vesicles (EVs) are gaining interest as efficient, biocompatible vehicles for cellular (show more...)
EV-METRIC
50% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
PEI precipitation
Protein markers
EV: CD9/ CD63/ Flotillin-1/ HSP70/ GFP
non-EV: GRP94 Proteomics
no
Show all info
Study aim
Mechanism of uptake/transfer/New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
HEK293
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
Other
Name other separation method
PEI precipitation
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ Flotillin-1/ HSP70
Not detected EV-associated proteins
GFP
Not detected contaminants
GRP94
Flow cytometry
Type of Flow cytometry
Beckman Coulter Cytoflex
Hardware adaptation to ~100nm EV's
The better resolution of the CytoFLEX is reached by using the violet side scatter of the 405 nm laser (manually set to 1600 and height threshold) and by performing preanalytical preparations with Fluorescent Megamix-Plus SSC beads (Cosmo Bio Co., LTD, Japan) which are FITC-labeled beads of increasing size (100, 160, 200, 240, 300, 500, 900 nm). beads were used to set the EV gate and manual gating was set to the populations of interest with reference to a negative control sample (GFP- EVs from HEK293 cells)
Calibration bead size
0.1/ 0.16/ 0.2/ 0.24/ 0.3/ 0.5/ 0.9
Antibody details provided?
No
Not detected EV-associated proteins
GFP
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV240006 | 1/4 | Mus musculus | Neuro-2a (N2a) CCL-131 |
(d)(U)C Total Exosome Isolation lipid-based affinity capture |
Weerakkody, Jonathan S. | 2024 | 44% | |
Study summaryFull title
All authors
Jonathan S. Weerakkody, Tiffany Tseng, Mackenzie Topper, Sikha Thoduvayil, Abhijith Radhakrishnan, Frederic Pincet, Themis R. Kyriakides, Roshan W. Gunasekara, Sathish Ramakrishnan
Journal
Abstract
The biggest challenge in current isolation methods for lipid bilayer-encapsulated vesicles, such as (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
small extracellular vesicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Total Exosome Isolation lipid-based affinity capture Protein markers
EV: CD9/ CD63/ CD81/ Syt1
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
Neuro-2a (N2a) CCL-131
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: rotor type
SW 55 Ti
Pelleting: speed (g)
12500
Commercial kit
Total Exosome Isolation
Other
Name other separation method
Total Exosome Isolation
Other
Name other separation method
lipid-based affinity capture
EV-subtype
Distinction between multiple subtypes
Size
Used subtypes
30-150
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81/ Syt1
Detected EV-associated proteins
CD9/ CD63/ Syt1
Characterization: RNA analysis
RNA analysis
Type
RNA-sequencing
Proteinase treatment
No
RNAse treatment
No
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
100-150
Extra information
This paper was to validate the efficacy of photosensitive lipid nanoprobe for the isolation and size selective enrichment of native extracellular vesicles
|
||||||||
EV240006 | 2/4 | Mus musculus | Primary bone marrow-derived macrophage (BMDM) cells from hind limbs in C57BL/6J wild type mice |
(d)(U)C Total Exosome Isolation lipid-based affinity capture |
Weerakkody, Jonathan S. | 2024 | 44% | |
Study summaryFull title
All authors
Jonathan S. Weerakkody, Tiffany Tseng, Mackenzie Topper, Sikha Thoduvayil, Abhijith Radhakrishnan, Frederic Pincet, Themis R. Kyriakides, Roshan W. Gunasekara, Sathish Ramakrishnan
Journal
Abstract
The biggest challenge in current isolation methods for lipid bilayer-encapsulated vesicles, such as (show more...)
EV-METRIC
44% (84th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Total Exosome Isolation lipid-based affinity capture Protein markers
EV: CD9/ CD63/ CD81/ Syt1
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-producing cells
Primary bone marrow-derived macrophage (BMDM) cells from hind limbs in C57BL/6J wild type mice
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
Commercial EDS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: rotor type
SW 55 Ti
Pelleting: speed (g)
12500
Commercial kit
Total Exosome Isolation
Other
Name other separation method
Total Exosome Isolation
Other
Name other separation method
lipid-based affinity capture
EV-subtype
Distinction between multiple subtypes
Size
Used subtypes
30-200
Characterization: Protein analysis
Protein Concentration Method
Not determined
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ CD81/ Syt1
Detected EV-associated proteins
CD9/ CD63/ Syt1
Characterization: Lipid analysis
No
Characterization: Particle analysis
DLS
Report type
Mean
Reported size (nm)
220-350
Extra information
This paper was to validate the efficacy of photosensitive lipid nanoprobe for the isolation and size selective enrichment of native extracellular vesicles
|
||||||||
EV230985 | 1/5 | Capra hircus | Milk |
(d)(U)C Filtration |
Santoro J | 2024 | 44% | |
Study summaryFull title
All authors
Santoro J, Nuzzo S, Franzese M, Salvatore M, Grimaldi AM
Journal
Heliyon
Abstract
Goat milk is a complex biological fluid, which in addition to having a high nutritional value, it is (show more...)
EV-METRIC
44% (70th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Milk
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: CD81/ TSG101
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Capra hircus
Sample Type
Milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: rotor type
MLA-80
Pelleting: speed (g)
200000
Wash: volume per pellet (ml)
5
Wash: time (min)
90
Wash: Rotor Type
MLA-80
Wash: speed (g)
200000
Filtration steps
Between 0.22 and 0.45 ?m/ 0.2 or 0.22 ?m
Size-exclusion chromatography
Resin type
Characterization: Protein analysis
Protein Concentration Method
Bradford
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ TSG101
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
161.1
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 5.60E+12
|
||||||||
EV230985 | 2/5 | Capra hircus | Milk |
(d)(U)C EDTA pretreatment |
Santoro J | 2024 | 44% | |
Study summaryFull title
All authors
Santoro J, Nuzzo S, Franzese M, Salvatore M, Grimaldi AM
Journal
Heliyon
Abstract
Goat milk is a complex biological fluid, which in addition to having a high nutritional value, it is (show more...)
EV-METRIC
44% (70th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Milk
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
EDTA pretreatment Protein markers
EV: CD81/ TSG101
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Capra hircus
Sample Type
Milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: rotor type
MLA-80
Pelleting: speed (g)
200000
Wash: volume per pellet (ml)
5
Wash: time (min)
90
Wash: Rotor Type
MLA-80
Wash: speed (g)
200000
Filtration steps
Between 0.22 and 0.45 ?m/ 0.2 or 0.22 ?m
Size-exclusion chromatography
Resin type
Other
Name other separation method
EDTA pretreatment
Characterization: Protein analysis
Protein Concentration Method
Bradford
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ TSG101
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
179.2
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.44E+11
|
||||||||
EV230985 | 3/5 | Capra hircus | Milk |
(d)(U)C HCl pretreatment |
Santoro J | 2024 | 44% | |
Study summaryFull title
All authors
Santoro J, Nuzzo S, Franzese M, Salvatore M, Grimaldi AM
Journal
Heliyon
Abstract
Goat milk is a complex biological fluid, which in addition to having a high nutritional value, it is (show more...)
EV-METRIC
44% (70th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Milk
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
HCl pretreatment Protein markers
EV: CD81/ TSG101
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Capra hircus
Sample Type
Milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: rotor type
MLA-80
Pelleting: speed (g)
200000
Wash: volume per pellet (ml)
5
Wash: time (min)
90
Wash: Rotor Type
MLA-80
Wash: speed (g)
200000
Filtration steps
Between 0.22 and 0.45 ?m/ 0.2 or 0.22 ?m
Size-exclusion chromatography
Resin type
Other
Name other separation method
HCl pretreatment
Characterization: Protein analysis
Protein Concentration Method
Bradford
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ TSG101
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
122.6
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 4.20E+10
|
||||||||
EV230372 | 14/14 | Homo sapiens | Blood plasma | (d)(U)C | Schöne N | 2024 | 43% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
43% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NSCLC
Focus vesicles
small EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
143000
Wash: volume per pellet (ml)
1.4
Wash: time (min)
90
Wash: Rotor Type
TLA-55
Wash: speed (g)
143000
Characterization: Protein analysis
None
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
143.7
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 2.85E+08
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
|
||||||||
EV240036 | 1/6 | Homo sapiens | Serum | Exoquick | Shinde U | 2024 | 38% | |
Study summaryFull title
All authors
Shinde U, Rao A, Bansal V, Das DK, Balasinor NH, Madan T
Journal
Reproduction
Abstract
Circulating extracellular vesicles of placental/amniochorionic origin carry placental/amniochorionic (show more...)
EV-METRIC
38% (82nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Non pregnant
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Commercial method
Protein markers
EV: CD9/ CD63/ PLAP/ Cullin 7
non-EV: None Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
Commercial kit
Exoquick
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9/ CD63/ PLAP/ Cullin 7
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV240036 | 3/6 | Homo sapiens | Serum |
Exoquick IAF |
Shinde U | 2024 | 38% | |
Study summaryFull title
All authors
Shinde U, Rao A, Bansal V, Das DK, Balasinor NH, Madan T
Journal
Reproduction
Abstract
Circulating extracellular vesicles of placental/amniochorionic origin carry placental/amniochorionic (show more...)
EV-METRIC
38% (82nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
Non pregnant
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
Commercial method
Immunoaffinity capture (non-commercial) Protein markers
EV: PLAP/ HLA-G
non-EV: Calnexin Proteomics
no
Show all info
Study aim
New methodological development
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
Commercial kit
Exoquick
Immunoaffinity capture
Selected surface protein(s)
PLAP/ HLA-G
EV-subtype
Distinction between multiple subtypes
affinity capture
Used subtypes
PLAP+ HLA-G+
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
PLAP/ HLA-G
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
None
|
||||||||
EV230985 | 4/5 | Capra hircus | Milk |
(d)(U)C SEC (non-commercial) Filtration EDTA pretreatment |
Santoro J | 2024 | 38% | |
Study summaryFull title
All authors
Santoro J, Nuzzo S, Franzese M, Salvatore M, Grimaldi AM
Journal
Heliyon
Abstract
Goat milk is a complex biological fluid, which in addition to having a high nutritional value, it is (show more...)
EV-METRIC
38% (58th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Milk
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Size-exclusion chromatography (non-commercial) Filtration EDTA pretreatment Protein markers
EV: CD81/ TSG101
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Capra hircus
Sample Type
Milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
No
Filtration steps
Between 0.22 and 0.45 ?m/ 0.2 or 0.22 ?m
Size-exclusion chromatography
Total column volume (mL)
2
Sample volume/column (mL)
2
Other
Name other separation method
EDTA pretreatment
Characterization: Protein analysis
Protein Concentration Method
Bradford
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ TSG101
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
179.4
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 1.88E+10
|
||||||||
EV230985 | 5/5 | Capra hircus | Milk |
(d)(U)C SEC (non-commercial) Filtration HCl pretreatment |
Santoro J | 2024 | 38% | |
Study summaryFull title
All authors
Santoro J, Nuzzo S, Franzese M, Salvatore M, Grimaldi AM
Journal
Heliyon
Abstract
Goat milk is a complex biological fluid, which in addition to having a high nutritional value, it is (show more...)
EV-METRIC
38% (58th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Milk
Sample origin
Control condition
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Size-exclusion chromatography (non-commercial) Filtration HCl pretreatment Protein markers
EV: CD81/ TSG101
non-EV: Calnexin Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Capra hircus
Sample Type
Milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
No
Filtration steps
Between 0.22 and 0.45 ?m/ 0.2 or 0.22 ?m
Size-exclusion chromatography
Total column volume (mL)
2
Sample volume/column (mL)
2
Other
Name other separation method
HCl pretreatment
Characterization: Protein analysis
Protein Concentration Method
Bradford
Protein Yield (µg)
per milliliter of starting sample
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ TSG101
Not detected contaminants
Calnexin
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
170.8
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 7.60E+10
|
||||||||
EV230994 | 1/6 | Bos taurus | Commercially available FBS | (d)(U)C | Urzì O | 2024 | 29% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
29% (25th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Commercially available FBS
Sample origin
no-HI
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Bos taurus
Sample Type
Commercially available FBS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
16500
Characterization: Protein analysis
None
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 74680000
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230994 | 3/6 | Bos taurus | Commercially available FBS | (d)(U)C | Urzì O | 2024 | 29% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
29% (25th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Commercially available FBS
Sample origin
HI-after EV-depl
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Bos taurus
Sample Type
Commercially available FBS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
16500
Characterization: Protein analysis
None
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 21600000
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV230994 | 5/6 | Bos taurus | Commercially available FBS | (d)(U)C | Urzì O | 2024 | 29% | |
Study summaryFull title
All authors
Urzì O, Bergqvist M, Lässer C, Moschetti M, Johansson J, D Arrigo D, Olofsson Bagge R, Crescitelli R
Journal
J Extracell Vesicles
Abstract
The release of extracellular vesicles (EVs) in cell cultures as well as their molecular cargo can be (show more...)
EV-METRIC
29% (25th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Commercially available FBS
Sample origin
HI-before EV-depl
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Technical analysis comparing/optimizing EV-related methods
Sample
Species
Bos taurus
Sample Type
Commercially available FBS
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
Type 45 Ti
Pelleting: speed (g)
118000
Characterization: Protein analysis
None
Protein Concentration Method
Fluorometric assay
Protein Yield (µg)
per milliliter of starting sample
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 24400000
EM
EM-type
Transmission-EM
Image type
Close-up
|
||||||||
EV240020 | 1/2 | Escherichia coli | REL606 |
(d)(U)C Filtration |
Schaack B | 2024 | 22% | |
Study summaryFull title
All authors
Schaack B, Mercier C, Katby M, Hannani D, Vollaire J, Robert JS, Caffaratti C, Blanquet F, Nicoud O, Josserand V, Laurin D
Journal
Int J Mol Sci
Abstract
A cell's ability to secrete extracellular vesicles (EVs) for communication is present in all three d (show more...)
EV-METRIC
22% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
Outer membrane vasicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Filtration Protein markers
EV: OmpA
non-EV: None Proteomics
no
Show all info
Study aim
Mechanism of uptake/transfer
Sample
Species
Escherichia coli
Sample Type
Cell culture supernatant
EV-producing cells
REL606
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 50.2 Ti
Pelleting: speed (g)
100000
Filtration steps
0.2 or 0.22 µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
OmpA
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
56
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
56
|
||||||||
EV240020 | 2/2 | Escherichia coli | REL606 | (d)(U)C | Schaack B | 2024 | 22% | |
Study summaryFull title
All authors
Schaack B, Mercier C, Katby M, Hannani D, Vollaire J, Robert JS, Caffaratti C, Blanquet F, Nicoud O, Josserand V, Laurin D
Journal
Int J Mol Sci
Abstract
A cell's ability to secrete extracellular vesicles (EVs) for communication is present in all three d (show more...)
EV-METRIC
22% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
miRFP713-expressing
Focus vesicles
Outer membrane vasicles
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: OmpA
non-EV: None Proteomics
no
Show all info
Study aim
Mechanism of uptake/transfer
Sample
Species
Escherichia coli
Sample Type
Cell culture supernatant
EV-producing cells
REL606
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
Type 50.2 Ti
Pelleting: speed (g)
100000
Filtration steps
0.2 or 0.22 µm
Characterization: Protein analysis
Protein Concentration Method
BCA
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
OmpA
Characterization: Lipid analysis
Yes
Characterization: Particle analysis
DLS
Report type
Size range/distribution
Reported size (nm)
56
EM
EM-type
Transmission-EM
Image type
Close-up
Report size (nm)
56
|
||||||||
EV230572 | 5/6 | Homo sapiens | SUM159-DSP1-CD63/DSP2 | (d)(U)C | Djeungoue-Petga, Marie-Ange | 2024 | 14% | |
Study summaryFull title
All authors
Marie Ange Djeungoue Petgaa, Catherine Taylora, Alexander Macpherson, Surendar Reddy Dhadi, Thomas Rollin, Jeremy W. Roya, Anirban Ghosh, Stephen M. Lewis, Rodney J. Ouellette
Journal
Abstract
Extracellular vesicles (EVs) are gaining interest as efficient, biocompatible vehicles for cellular (show more...)
EV-METRIC
14% (44th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
DSP1-CD63/DSP2 overexpression
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Mechanism of uptake/transfer/New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
SUM159-DSP1-CD63/DSP2
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 100,000 g and 150,000 g
Pelleting performed
Yes
Pelleting: rotor type
SW 40 Ti
Pelleting: speed (g)
100000
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
~180
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 5-7E07
|
||||||||
EV230372 | 12/14 | Homo sapiens | Blood plasma | (d)(U)C | Schöne N | 2024 | 14% | |
Study summaryFull title
All authors
Schöne N, Kemper M, Menck K, Evers G, Krekeler C, Schulze AB, Lenz G, Wardelmann E, Binder C, Bleckmann A
Journal
J Extracell Vesicles
Abstract
Immunotherapy has revolutionized the treatment of patients with non-small cell lung cancer (NSCLC). (show more...)
EV-METRIC
14% (38th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
Control condition
Focus vesicles
small EVs
Separation protocol
Separation protocol
(Differential) (ultra)centrifugation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: rotor type
SW 32 Ti
Pelleting: speed (g)
143000
Wash: volume per pellet (ml)
1.4
Wash: time (min)
90
Wash: Rotor Type
TLA-55
Wash: speed (g)
143000
Characterization: Protein analysis
None
Protein Concentration Method
Lowry-based assay
Protein Yield (µg)
per milliliter of starting sample
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Mean
Reported size (nm)
137.7
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 2.41E+08
|
||||||||
EV230572 | 6/6 | Homo sapiens | SUM159-DSP1-CD63/DSP2 | PEI precipitation | Djeungoue-Petga, Marie-Ange | 2024 | 0% | |
Study summaryFull title
All authors
Marie Ange Djeungoue Petgaa, Catherine Taylora, Alexander Macpherson, Surendar Reddy Dhadi, Thomas Rollin, Jeremy W. Roya, Anirban Ghosh, Stephen M. Lewis, Rodney J. Ouellette
Journal
Abstract
Extracellular vesicles (EVs) are gaining interest as efficient, biocompatible vehicles for cellular (show more...)
EV-METRIC
0% (median: 14% of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
DSP1-CD63/DSP2 overexpression
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
PEI precipitation
Protein markers
EV: None
non-EV: None Proteomics
no
Show all info
Study aim
Mechanism of uptake/transfer/New methodological development/Technical analysis comparing/optimizing EV-related methods
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
SUM159-DSP1-CD63/DSP2
EV-harvesting Medium
EV-depleted medium
Preparation of EDS
>=18h at >= 100,000g
Separation Method
Other
Name other separation method
PEI precipitation
Characterization: Protein analysis
None
Protein Concentration Method
Not determined
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Size range/distribution
Reported size (nm)
200-300
EV concentration
Yes
Particle yield
particles per milliliter of starting sample: 2-3.5E06
|
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