Search > Results
You searched for: 2013 (Year of publication)
Showing 51 - 100 of 384
Showing 51 - 100 of 384
Details | EV-TRACK ID | Experiment nr. | Species | Sample type | Separation protocol | First author | Year | EV-METRIC |
---|---|---|---|---|---|---|---|---|
EV130049 | 1/1 | Homo sapiens | Pleural effusion |
(d)(U)C DG |
Park JO | 2013 | 38% | |
Study summaryFull title
All authors
Park JO, Choi DY, Choi DS, Kim HJ, Kang JW, Jung JH, Lee JH, Kim J, Freeman MR, Lee KY, Gho YS, Kim KP
Journal
Proteomics
Abstract
Microvesicles (MVs, also known as exosomes, ectosomes, microparticles) are released by various cance (show more...)
EV-METRIC
38% (59th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Pleural effusion
Sample origin
NAY
Focus vesicles
microvesicles
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Ezrin/ Beta-actin/ HSP90/ CD63
non-EV: Proteomics
yes
EV density (g/ml)
1.100
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Pleural effusion
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Density gradient
Lowest density fraction
5
Highest density fraction
30
Orientation
Bottom-up
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ HSP90/ Beta-actin/ Ezrin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Beta-actin/ Ezrin
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV130101 | 1/2 | Homo sapiens | NAY |
(d)(U)C Filtration |
Marimpietri D | 2013 | 38% | |
Study summaryFull title
All authors
Marimpietri D, Petretto A, Raffaghello L, Pezzolo A, Gagliani C, Tacchetti C, Mauri P, Melioli G, Pistoia V
Journal
PLoS One
Abstract
Neuroblastoma (NB) is the most common extracranial solid tumor in childhood, with grim prognosis in (show more...)
EV-METRIC
38% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
156.9 (pelleting) / 156.9 (washing)
Protein markers
EV: CD81/ Fibronectin/ CD63/ CD9
non-EV: Proteomics
yes
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
75
Pelleting: rotor type
70Ti
Pelleting: adjusted k-factor
156.9
Wash: Rotor Type
70Ti
Wash: adjusted k-factor
156.9
Filtration steps
0.22µm or 0.2µm0.2µm > x > 0.1µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Fibronectin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Fibronectin
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV130100 | 1/2 | Rattus norvegicus/rattus | NAY |
ExoQuick UF |
Malik ZA | 2013 | 38% | |
Study summaryFull title
All authors
Malik ZA, Kott KS, Poe AJ, Kuo T, Chen L, Ferrara KW, Knowlton AA
Journal
Am J Physiol Heart Circ Physiol
Abstract
Exosomes, which are 50- to 100-nm-diameter lipid vesicles, have been implicated in intercellular com (show more...)
EV-METRIC
38% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
ExoQuick
UF Protein markers
EV: AChE/ HSP60/ GAPDH
non-EV: Proteomics
yes
Show all info
Study aim
Omics
Sample
Species
Rattus norvegicus/rattus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
Commercial kit
ExoQuick
Other
Name other separation method
ExoQuick
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
HSP60/ GAPDH/ AChE
ELISA
Antibody details provided?
No
Detected EV-associated proteins
HSP60/ GAPDH/ AChE
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV130040 | 1/1 | Homo sapiens | Blood plasma | DG | Gourzones C | 2013 | 38% | |
Study summaryFull title
All authors
Gourzones C, Ferrand FR, Amiel C, Vérillaud B, Barat A, Guérin M, Gattolliat CH, Gelin A, Klibi J, Chaaben AB, Schneider V, Guemira F, Guigay J, Lang P, Jimenez-Pailhes AS, Busson P
Journal
Virol J
Abstract
BACKGROUND: Because latent Epstein Barr (EBV)-infection is a specific characteristic of malignant na (show more...)
EV-METRIC
38% (72nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG
Protein markers
EV: CD63
non-EV: Proteomics
no
EV density (g/ml)
1.15-1.19
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
Density gradient
Lowest density fraction
1.006g/L
Highest density fraction
1.24g/L
Orientation
Bottom-up
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63
Characterization: Particle analysis
None
|
||||||||
EV130026 | 2/3 | Homo sapiens | Urine |
(d)(U)C UF |
Gerlach JQ | 2013 | 38% | |
Study summaryFull title
All authors
Gerlach JQ, Krüger A, Gallogly S, Hanley SA, Hogan MC, Ward CJ, Joshi L, Griffin MD
Journal
PLoS One
Abstract
Urinary extracellular vesicles (uEVs) are released by cells throughout the nephron and contain biomo (show more...)
EV-METRIC
38% (73rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
UF Protein markers
EV: AQP2/ CD24
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Pelleting performed
No
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD24/ AQP2
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD24/ AQP2
Characterization: Particle analysis
TRPS
EM
EM-type
transmission EM
|
||||||||
EV130034 | 1/1 | Homo sapiens | Semen |
(d)(U)C DG SEC |
Brouwers JF | 2013 | 38% | |
Study summaryFull title
All authors
Brouwers JF, Aalberts M, Jansen JW, van Niel G, Wauben MH, Stout TA, Helms JB, Stoorvogel W
Journal
Proteomics
Abstract
Prostasomes are vesicles secreted by prostate epithelial cells and found in abundance in seminal pla (show more...)
EV-METRIC
38% (68th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Semen
Sample origin
NAY
Focus vesicles
Prostasomes
Separation protocol
Separation protocol
(d)(U)C
DG SEC Protein markers
EV: CD9
non-EV: PSCA Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Semen
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Pelleting performed
No
Density gradient
Lowest density fraction
0.4
Highest density fraction
2
Orientation
Top-down
Speed (g)
100000
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9
Detected contaminants
PSCA
Characterization: Particle analysis
EM
EM-type
cryo EM
Image type
Close-up
|
||||||||
EV130032 | 1/1 | Homo sapiens | NAY |
(d)(U)C DG Filtration UF |
Bernhard OK | 2013 | 38% | |
Study summaryFull title
All authors
Bernhard OK, Greening DW, Barnes TW, Ji H, Simpson RJ
Journal
Biochim Biophys Acta Proteins Proteom
Abstract
Colorectal cancer (CRC), one of the most prevalent cancers in the western world, is treatable if det (show more...)
EV-METRIC
38% (79th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Filtration UF Protein markers
EV: CD9
non-EV: Proteomics
no
EV density (g/ml)
1.110
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Pelleting performed
No
Density gradient
Lowest density fraction
5
Highest density fraction
40
Orientation
Top-down
Speed (g)
100000
Filtration steps
0.2µm > x > 0.1µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9
Characterization: Particle analysis
None
|
||||||||
EV130135 | 2/2 | Homo sapiens | NAY |
(d)(U)C DG |
Lee JH | 2013 | 33% | |
Study summaryFull title
All authors
Lee JH, Wittki S, Bräu T, Dreyer FS, Krätzel K, Dindorf J, Johnston IC, Gross S, Kremmer E, Zeidler R, Schlötzer-Schrehardt U, Lichtenheld M, Saksela K, Harrer T, Schuler G, Federico M, Baur AS
Journal
Mol Cell
Abstract
The HIV Nef protein recruits the polycomb protein Eed and mimics an integrin receptor signal for rea (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: CD81/ ADAM17/ CD63/ CD9/ MHC1
non-EV: Proteomics
no
EV density (g/ml)
1.12-1.23
Show all info
Study aim
Other/Regulation of ADAM10 activation and secretion
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Wash: volume per pellet (ml)
35
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Bottom-up
Speed (g)
110000
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ CD9/ MHC1/ ADAM17
ELISA
Antibody details provided?
No
Detected EV-associated proteins
MHC1/ ADAM17
Characterization: Particle analysis
EM
EM-type
transmission EM/ scanning EM
Image type
Wide-field
Report size (nm)
Not reported
|
||||||||
EV130027 | 1/4 | Mus musculus | NAY | (d)(U)C | Hajj GN | 2013 | 33% | |
Study summaryFull title
All authors
Hajj GN, Arantes CP, Dias MV, Roffé M, Costa-Silva B, Lopes MH, Porto-Carreiro I, Rabachini T, Lima FR, Beraldo FH, Prado MA, Linden R, Martins VR
Journal
Cell Mol Life Sci
Abstract
The co-chaperone stress-inducible protein 1 (STI1) is released by astrocytes, and has important neur (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ Flotilin1/ VPS36/ Tf-receptor/ HSP90/ PrP/ HSP70
non-EV: GAPDH/ LAMP1 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotilin1/ HSP90/ HSP70/ TSG101/ PrP/ Tf-receptor/ VPS36
Detected contaminants
"GAPDH/ LAMP1"
ELISA
Antibody details provided?
No
Detected EV-associated proteins
PrP/ Tf-receptor/ VPS36
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV130027 | 2/4 | Mus musculus | NAY | (d)(U)C | Hajj GN | 2013 | 33% | |
Study summaryFull title
All authors
Hajj GN, Arantes CP, Dias MV, Roffé M, Costa-Silva B, Lopes MH, Porto-Carreiro I, Rabachini T, Lima FR, Beraldo FH, Prado MA, Linden R, Martins VR
Journal
Cell Mol Life Sci
Abstract
The co-chaperone stress-inducible protein 1 (STI1) is released by astrocytes, and has important neur (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ Flotilin1/ VPS36/ Tf-receptor/ HSP90/ PrP/ HSP70
non-EV: GAPDH/ LAMP1 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
960
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotilin1/ HSP90/ HSP70/ TSG101/ PrP/ Tf-receptor/ VPS36
Detected contaminants
"GAPDH/ LAMP1"
ELISA
Antibody details provided?
No
Detected EV-associated proteins
PrP/ Tf-receptor/ VPS36
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV130027 | 3/4 | Mus musculus | NAY | (d)(U)C | Hajj GN | 2013 | 33% | |
Study summaryFull title
All authors
Hajj GN, Arantes CP, Dias MV, Roffé M, Costa-Silva B, Lopes MH, Porto-Carreiro I, Rabachini T, Lima FR, Beraldo FH, Prado MA, Linden R, Martins VR
Journal
Cell Mol Life Sci
Abstract
The co-chaperone stress-inducible protein 1 (STI1) is released by astrocytes, and has important neur (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: TSG101/ Flotilin1/ VPS36/ Tf-receptor/ HSP90/ PrP/ HSP70
non-EV: GAPDH/ LAMP1 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotilin1/ HSP90/ HSP70/ TSG101/ PrP/ Tf-receptor/ VPS36
Detected contaminants
"GAPDH/ LAMP1"
ELISA
Antibody details provided?
No
Detected EV-associated proteins
PrP/ Tf-receptor/ VPS36
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV130072 | 2/4 | Homo sapiens | NAY |
(d)(U)C DG |
Zeringer E | 2013 | 33% | |
Study summaryFull title
All authors
Zeringer E, Li M, Barta T, Schageman J, Pedersen KW, Neurauter A, Magdaleno S, Setterquist R, Vlassov AV
Journal
World J Methodol
Abstract
AIM: To develop protocols for isolation of exosomes and characterization of their RNA content. METHO (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: CD63
non-EV: Proteomics
no
EV density (g/ml)
1.13-1.19
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Density gradient
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Top-down
Speed (g)
110000
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63
Characterization: Particle analysis
NTA
|
||||||||
EV130071 | 2/2 | Bos bovis | Milk |
(d)(U)C DG Filtration |
Yamada T | 2013 | 33% | |
Study summaryFull title
All authors
Yamada T, Shigemura H, Ishiguro N, Inoshima Y
Journal
PLoS One
Abstract
Exosomes are small membranous microvesicles (40-100 nm in diameter) and are extracellularly released (show more...)
EV-METRIC
33% (53rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Milk
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV: MFGE8
non-EV: Proteomics
no
EV density (g/ml)
1.09-1.19
Show all info
Study aim
Omics
Sample
Species
Bos bovis
Sample Type
Milk
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
5
Highest density fraction
40
Orientation
Top-down
Speed (g)
100000
Filtration steps
0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
MFGE8
ELISA
Antibody details provided?
No
Detected EV-associated proteins
MFGE8
Characterization: Particle analysis
None
|
||||||||
EV130059 | 1/1 | Homo sapiens | NAY |
(d)(U)C DG |
van Balkom BW | 2013 | 33% | |
Study summaryFull title
All authors
van Balkom BW, de Jong OG, Smits M, Brummelman J, den Ouden K, de Bree PM, van Eijndhoven MA, Pegtel DM, Stoorvogel W, Würdinger T, Verhaar MC
Journal
Blood
Abstract
Signaling between endothelial cells, endothelial progenitor cells, and stromal cells is crucial for (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Flotilin1
non-EV: Proteomics
no
EV density (g/ml)
1.110
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Bottom-up
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotilin1
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV130121 | 1/2 | Homo sapiens | NAY | DG | Tan SS | 2013 | 33% | |
Study summaryFull title
All authors
Tan SS, Yin Y, Lee T, Lai RC, Yeo RW, Zhang B, Choo A, Lim SK
Journal
J Extracell Vesicles
Abstract
BACKGROUND: Mesenchymal stem cell (MSC) was previously shown to secrete lipid vesicles that when pur (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
DG
Protein markers
EV: CD81
non-EV: Proteomics
no
EV density (g/ml)
1.09-1.17
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
Density gradient
Lowest density fraction
22.8
Highest density fraction
60
Orientation
Top-down
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Selected surface protein(s)
Yes
Characterization: Particle analysis
None
|
||||||||
EV130118 | 1/1 | Homo sapiens | Blood plasma |
(d)(U)C Filtration |
Szajnik M | 2013 | 33% | |
Study summaryFull title
All authors
Szajnik M, Derbis M, Lach M, Patalas P, Michalak M, Drzewiecka H, Szpurek D, Nowakowski A, Spaczynski M, Baranowski W, Whiteside TL
Journal
Gynecol Obstet (Sunnyvale)
Abstract
BACKGROUND: In patients with Ovarian Cancer (OvCa) exosomes released by tumor cells are present in t (show more...)
EV-METRIC
33% (66th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
101.8 (pelleting)
Protein markers
EV: LAMP1
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
70.1Ti
Pelleting: adjusted k-factor
101.8
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
LAMP1
ELISA
Antibody details provided?
No
Detected EV-associated proteins
LAMP1
Characterization: Particle analysis
EM
EM-type
scanning EM
Image type
Wide-field
Report size (nm)
Not reported
|
||||||||
EV130019 | 1/1 | Homo sapiens | NAY | (d)(U)C | Svensson KJ | 2013 | 33% | |
Study summaryFull title
All authors
Svensson KJ, Christianson HC, Wittrup A, Bourseau-Guilmain E, Lindqvist E, Svensson LM, Mörgelin M, Belting M
Journal
J Biol Chem
Abstract
The role of exosomes in cancer can be inferred from the observation that they transfer tumor cell de (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Flotilin1/ CD63
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ Flotilin1
Detected contaminants
Cell organelle protein
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM/ immune EM
EM protein
Tsg101; Flotillin1
Image type
Close-up
|
||||||||
EV130057 | 1/1 | Homo sapiens | Placental explant |
(d)(U)C DG Filtration |
Stenqvist AC | 2013 | 33% | |
Study summaryFull title
All authors
Stenqvist AC, Nagaeva O, Baranov V, Mincheva-Nilsson L
Journal
J Immunol
Abstract
Apoptosis is crucially important in mediating immune privilege of the fetus during pregnancy. We inv (show more...)
EV-METRIC
33% (62nd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Placental explant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV: CD63
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Placental explant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Density gradient
Lowest density fraction
20
Highest density fraction
40
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Selected surface protein(s)
Yes
Characterization: Particle analysis
EM
EM-type
transmission EM/ immune EM
EM protein
CD63
Image type
Close-up, Wide-field
|
||||||||
EV130056 | 1/2 | Bos bovis | Follicular fluid |
(d)(U)C Filtration |
Sohel MM | 2013 | 33% | |
Study summaryFull title
All authors
Sohel MM, Hoelker M, Noferesti SS, Salilew-Wondim D, Tholen E, Looft C, Rings F, Uddin MJ, Spencer TE, Schellander K, Tesfaye D
Journal
PLoS One
Abstract
Cell-cell communication within the follicle involves many signaling molecules, and this process may (show more...)
EV-METRIC
33% (77th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Follicular fluid
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
138.6 (pelleting) / 138.6 (washing)
Protein markers
EV: CD63
non-EV: Cell organelle protein/ Ago2 Proteomics
no
Show all info
Study aim
Function
Sample
Species
Bos bovis
Sample Type
Follicular fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW55
Pelleting: adjusted k-factor
138.6
Wash: Rotor Type
SW55
Wash: adjusted k-factor
138.6
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63
Detected contaminants
Cell organelle protein/ Ago2
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV130114 | 1/3 | Homo sapiens | NAY | (d)(U)C | Shin SJ | 2013 | 33% | |
Study summaryFull title
All authors
Shin SJ, Smith JA, Rezniczek GA, Pan S, Chen R, Brentnall TA, Wiche G, Kelly KA
Journal
Proc Natl Acad Sci U S A
Abstract
We recently demonstrated that plectin is a robust biomarker for pancreatic ductal adenocarcinoma (PD (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
104.8 (pelleting)
Protein markers
EV: CD63/ CD81/ HSP90/ Alix/ HSP70/ CD9
non-EV: Proteomics
yes
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
960
Pelleting: rotor type
45Ti
Pelleting: adjusted k-factor
104.8
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD63/ CD81/ CD9/ HSP90/ HSP70
Characterization: Particle analysis
DLS
NTA
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV130055 | 1/1 | Homo sapiens | NAY |
(d)(U)C DC Filtration |
Salomon C | 2013 | 33% | |
Study summaryFull title
All authors
Salomon C, Ryan J, Sobrevia L, Kobayashi M, Ashman K, Mitchell M, Rice GE
Journal
PLoS One
Abstract
Vasculogenesis and angiogenesis are critical processes in fetal circulation and placental vasculatur (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DC Filtration Adj. k-factor
266.3 (pelleting)
Protein markers
EV: CD81/ CD63/ CD9
non-EV: Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
75
Pelleting: rotor type
Surespin630/36
Pelleting: adjusted k-factor
266.3
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ CD9
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV130054 | 1/1 | Homo sapiens | NAY | DG | Regev-Rudzki N | 2013 | 33% | |
Study summaryFull title
All authors
Regev-Rudzki N, Wilson DW, Carvalho TG, Sisquella X, Coleman BM, Rug M, Bursac D, Angrisano F, Gee M, Hill AF, Baum J, Cowman AF
Journal
Cell
Abstract
Cell-cell communication is an important mechanism for information exchange promoting cell survival f (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Exosome-like vesicles
Separation protocol
Separation protocol
DG
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
10
Highest density fraction
30
Orientation
Top-down
Characterization: Particle analysis
EM
EM-type
transmission EM/ cryo EM/ atomic force EM
Image type
Close-up, Wide-field
|
||||||||
EV130053 | 1/2 | Homo sapiens | NAY | (d)(U)C | Rappa G | 2013 | 33% | |
Study summaryFull title
All authors
Rappa G, Mercapide J, Anzanello F, Pope RM, Lorico A
Journal
Mol Cancer
Abstract
Exosomes can be viewed as complex messages packaged to survive trips to other cells in the local mic (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Alix
non-EV: Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
Alix
Characterization: Particle analysis
DLS
NTA
|
||||||||
EV130053 | 2/2 | Homo sapiens | NAY |
(d)(U)C Filtration IAF UF |
Rappa G | 2013 | 33% | |
Study summaryFull title
All authors
Rappa G, Mercapide J, Anzanello F, Pope RM, Lorico A
Journal
Mol Cancer
Abstract
Exosomes can be viewed as complex messages packaged to survive trips to other cells in the local mic (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration IAF UF Protein markers
EV: Alix
non-EV: Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Filtration steps
0.22µm or 0.2µm
Immunoaffinity capture
Selected surface protein(s)
prominin-1
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
Alix
Characterization: Particle analysis
DLS
NTA
|
||||||||
EV130153 | 2/3 | Homo sapiens | Urine |
(d)(U)C DG |
Oosthuyzen W | 2013 | 33% | |
Study summaryFull title
All authors
Oosthuyzen W, Sime NE, Ivy JR, Turtle EJ, Street JM, Pound J, Bath LE, Webb DJ, Gregory CD, Bailey MA, Dear JW
Journal
J Physiol
Abstract
Exosomes are vesicles that are released from the kidney into urine. They contain protein and RNA fro (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: TSG101/ Flotilin1/ CD24
non-EV: Proteomics
no
EV density (g/ml)
1.12-1.16
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Top-down
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotilin1/ TSG101/ CD24
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD24
Characterization: Particle analysis
None
|
||||||||
EV130065 | 3/3 | Homo sapiens | NAY |
(d)(U)C DG Filtration |
Kulshreshtha A | 2013 | 33% | |
Study summaryFull title
All authors
Kulshreshtha A, Ahmad T, Agrawal A, Ghosh B
Journal
J Allergy Clin Immunol
Abstract
BACKGROUND: Exosomes are nanovesicles involved in intercellular communication. Their roles in variou (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV: CD81/ CD63/ TSG101/ Annexin5/ HSP70
non-EV: Proteomics
no
EV density (g/ml)
1.16-1.21
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Top-down
Rotor type
SW50.1
Speed (g)
200000
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ HSP70/ TSG101/ Annexin5
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Annexin5
Characterization: Particle analysis
None
|
||||||||
EV130043 | 1/2 | Homo sapiens | NAY | (d)(U)C | Kucharzewska P | 2013 | 33% | |
Study summaryFull title
All authors
Kucharzewska P, Christianson HC, Welch JE, Svensson KJ, Fredlund E, Ringnér M, Mörgelin M, Bourseau-Guilmain E, Bengzon J, Belting M
Journal
Proc Natl Acad Sci U S A
Abstract
Hypoxia, or low oxygen tension, is a major regulator of tumor development and aggressiveness. Howeve (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD81/ CD63/ Flotillin
non-EV: Tubulin / Cell organelle protein Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ Flotillin
Detected contaminants
Cell organelle protein/ "Tubulin "
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Flotillin
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV130043 | 2/2 | Homo sapiens | Blood plasma | (d)(U)C | Kucharzewska P | 2013 | 33% | |
Study summaryFull title
All authors
Kucharzewska P, Christianson HC, Welch JE, Svensson KJ, Fredlund E, Ringnér M, Mörgelin M, Bourseau-Guilmain E, Bengzon J, Belting M
Journal
Proc Natl Acad Sci U S A
Abstract
Hypoxia, or low oxygen tension, is a major regulator of tumor development and aggressiveness. Howeve (show more...)
EV-METRIC
33% (66th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD81/ CAV1/ LAMP1
non-EV: Tubulin/ Cell organelle protein Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD81/ "LAMP1 / CAV1"
Detected contaminants
Cell organelle protein/ Tubulin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
"LAMP1 / CAV1"
Characterization: Particle analysis
NTA
|
||||||||
EV130073 | 3/5 | Homo sapiens | Blood plasma |
(d)(U)C Filtration IAF |
Kalra H | 2013 | 33% | |
Study summaryFull title
All authors
Kalra H, Adda CG, Liem M, Ang CS, Mechler A, Simpson RJ, Hulett MD, Mathivanan S
Journal
Proteomics
Abstract
Exosomes are nanovesicles released by a variety of cells and are detected in body fluids including b (show more...)
EV-METRIC
33% (66th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration IAF Protein markers
EV: TSG101/ CD63/ Flotilin1/ Alix/ TFRC/ HSP70/ CD9
non-EV: Proteomics
yes
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Filtration steps
0.2µm > x > 0.1µm
Immunoaffinity capture
Selected surface protein(s)
EpCAM
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD63/ CD9/ Flotilin1/ HSP70/ TSG101/ TFRC
ELISA
Antibody details provided?
No
Detected EV-associated proteins
TFRC
Characterization: Particle analysis
EM
EM-type
transmission EM/ atomic force EM
Image type
Close-up, Wide-field
|
||||||||
EV130073 | 4/5 | Homo sapiens | Blood plasma |
(d)(U)C Filtration |
Kalra H | 2013 | 33% | |
Study summaryFull title
All authors
Kalra H, Adda CG, Liem M, Ang CS, Mechler A, Simpson RJ, Hulett MD, Mathivanan S
Journal
Proteomics
Abstract
Exosomes are nanovesicles released by a variety of cells and are detected in body fluids including b (show more...)
EV-METRIC
33% (66th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: TSG101/ CD63/ Flotilin1/ Alix/ TFRC/ HSP70/ CD9
non-EV: Proteomics
yes
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Wash: volume per pellet (ml)
10
Filtration steps
0.2µm > x > 0.1µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD63/ CD9/ Flotilin1/ HSP70/ TSG101/ TFRC
ELISA
Antibody details provided?
No
Detected EV-associated proteins
TFRC
Characterization: Particle analysis
EM
EM-type
transmission EM/ atomic force EM
Image type
Close-up, Wide-field
|
||||||||
EV130011 | 1/1 | Homo sapiens | NAY |
(d)(U)C Filtration |
Kajimoto T | 2013 | 33% | |
Study summaryFull title
All authors
Kajimoto T, Okada T, Miya S, Zhang L, Nakamura S
Journal
Nat Commun
Abstract
During late endosome maturation, cargo molecules are sorted into intralumenal vesicles (ILVs) of mul (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Filtration Protein markers
EV: Tf-receptor/ CD81/ CD63/ HSP70
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD63/ CD81/ HSP70/ Tf-receptor
Detected contaminants
Cell organelle protein
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Tf-receptor
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Selected surface protein(s)
Yes
Characterization: Particle analysis
None
|
||||||||
EV130144 | 2/2 | Mus musculus | NAY |
(d)(U)C DG Filtration |
Hassani K | 2013 | 33% | |
Study summaryFull title
All authors
Hassani K, Olivier M
Journal
PLoS Negl Trop Dis
Abstract
Released by many eukaryotic cells, the exosomes are 40-100 nm vesicles shown to operate over the com (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV: Tubulin/ GP63/ PABP/ Actin/ PGK1
non-EV: Proteomics
yes
EV density (g/ml)
1.13-1.16
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Only used for validation of main results
Yes
Highest density fraction
2
Orientation
Top-down
Speed (g)
100000
Filtration steps
0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Actin/ GP63/ Tubulin/ PGK1/ PABP
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Actin/ GP63/ Tubulin/ PGK1/ PABP
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV130090 | 1/2 | Rattus norvegicus/rattus | NAY |
(d)(U)C DG |
Grapp M | 2013 | 33% | |
Study summaryFull title
All authors
Grapp M, Wrede A, Schweizer M, Hüwel S, Galla HJ, Snaidero N, Simons M, Bückers J, Low PS, Urlaub H, Gärtner J, Steinfeld R
Journal
Nat Commun
Abstract
Loss of folate receptor-? function is associated with cerebral folate transport deficiency and child (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Alix/ Flotillin2
non-EV: Proteomics
yes
EV density (g/ml)
1.13-1.16
Show all info
Study aim
Function
Sample
Species
Rattus norvegicus/rattus
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
150
Density gradient
Only used for validation of main results
Yes
Orientation
Top-down
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ Flotillin2
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Flotillin2
Characterization: Particle analysis
None
|
||||||||
EV130090 | 2/2 | Homo sapiens | "Cerebrospinal fluid" |
(d)(U)C DG |
Grapp M | 2013 | 33% | |
Study summaryFull title
All authors
Grapp M, Wrede A, Schweizer M, Hüwel S, Galla HJ, Snaidero N, Simons M, Bückers J, Low PS, Urlaub H, Gärtner J, Steinfeld R
Journal
Nat Commun
Abstract
Loss of folate receptor-? function is associated with cerebral folate transport deficiency and child (show more...)
EV-METRIC
33% (87th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
"Cerebrospinal fluid"
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Flotillin2
non-EV: Proteomics
yes
EV density (g/ml)
1.11-1.16
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
"Cerebrospinal fluid"
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
150
Density gradient
Only used for validation of main results
Yes
Orientation
Top-down
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin2
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Flotillin2
Characterization: Particle analysis
EM
EM-type
immune EM
EM protein
Flotillin2
Image type
Close-up
|
||||||||
EV130026 | 1/3 | Homo sapiens | Urine |
(d)(U)C UF |
Gerlach JQ | 2013 | 33% | |
Study summaryFull title
All authors
Gerlach JQ, Krüger A, Gallogly S, Hanley SA, Hogan MC, Ward CJ, Joshi L, Griffin MD
Journal
PLoS One
Abstract
Urinary extracellular vesicles (uEVs) are released by cells throughout the nephron and contain biomo (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
UF Protein markers
EV: AQP2/ CD24
non-EV: Proteomics
no
Show all info
Study aim
Technical
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
150
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
CD24/ AQP2
ELISA
Antibody details provided?
No
Detected EV-associated proteins
CD24/ AQP2
Characterization: Particle analysis
TRPS
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV130075 | 3/3 | Homo sapiens | Urine |
(d)(U)C DG |
Fraser KB | 2013 | 33% | |
Study summaryFull title
All authors
Fraser KB, Moehle MS, Daher JP, Webber PJ, Williams JY, Stewart CA, Yacoubian TA, Cowell RM, Dokland T, Ye T, Chen D, Siegal GP, Galemmo RA, Tsika E, Moore DJ, Standaert DG, Kojima K, Mobley JA, West AB
Journal
Hum Mol Genet
Abstract
Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene cause late-onset Parkinson's disease (PD) (show more...)
EV-METRIC
33% (65th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Urine
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Alix/ TSG101/ CD9
non-EV: Proteomics
yes
EV density (g/ml)
1.176
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Urine
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Density gradient
Only used for validation of main results
Yes
Speed (g)
500000
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD9/ TSG101
Characterization: Particle analysis
EM
EM-type
cryo EM
Image type
Wide-field
|
||||||||
EV130039 | 1/1 | Homo sapiens | NAY |
(d)(U)C DG |
Feng Z | 2013 | 33% | |
Study summaryFull title
All authors
Feng Z, Hensley L, McKnight KL, Hu F, Madden V, Ping L, Jeong SH, Walker C, Lanford RE, Lemon SM
Journal
Nature
Abstract
Animal viruses are broadly categorized structurally by the presence or absence of an envelope compos (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Alix/ Flotilin1
non-EV: Proteomics
no
EV density (g/ml)
1.100
Show all info
Study aim
Other/To decipher virus secretion into EVs
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Density gradient
Lowest density fraction
8
Highest density fraction
40
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ Flotilin1
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up, Wide-field
|
||||||||
EV130038 | 1/1 | Homo sapiens | NAY | (d)(U)C | Duijvesz D | 2013 | 33% | |
Study summaryFull title
All authors
Duijvesz D, Burnum-Johnson KE, Gritsenko MA, Hoogland AM, Vredenbregt-van den Berg MS, Willemsen R, Luider T, Paša-Tolic L, Jenster G
Journal
PLoS One
Abstract
BACKGROUND: Current markers for prostate cancer, such as PSA lack specificity. Therefore, novel biom (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Alix/ CD9
non-EV: Proteomics
yes
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 50,000 g and 100,000 g Pelleting performed
Yes
Pelleting: time(min)
110
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
Alix/ CD9
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV130142 | 1/2 | Homo sapiens | Serum |
(d)(U)C DG IAF |
Di Noto G | 2013 | 33% | |
Study summaryFull title
All authors
Di Noto G, Paolini L, Zendrini A, Radeghieri A, Caimi L, Ricotta D
Journal
PLoS One
Abstract
Plasma cell dyscrasias are immunosecretory disorders that can lead to hematological malignancies suc (show more...)
EV-METRIC
33% (76th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Serum
Sample origin
NAY
Focus vesicles
microvesicles
Separation protocol
Separation protocol
(d)(U)C
DG IAF Adj. k-factor
89.21 (pelleting)
Protein markers
EV: Tubulin/ HSP70/ Annexin5
non-EV: Proteomics
no
Show all info
Study aim
Biomarker
Sample
Species
Homo sapiens
Sample Type
Serum
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
TLA55
Pelleting: adjusted k-factor
89.21
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
15
Highest density fraction
60
Orientation
Top-down
Immunoaffinity capture
Selected surface protein(s)
Annexin5
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
HSP70/ Annexin5/ Tubulin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Annexin5/ Tubulin
Characterization: Particle analysis
EM
EM-type
scanning EM
Image type
Wide-field
Report size (nm)
Not reported
|
||||||||
EV130004 | 2/3 | Homo sapiens | DLD-1 |
(d)(U)C UF Filtration |
Demory Beckler M | 2013 | 33% | |
Study summaryFull title
All authors
Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ
Journal
Mol Cell Proteomics
Abstract
Activating mutations in KRAS occur in 30% to 40% of colorectal cancers. How mutant KRAS alters cance (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
Control condition
Focus vesicles
exosome
Separation protocol
Separation protocol
(d)(U)C
UF Filtration Protein markers
EV: "Flotillin1/ TSG101/ HSP70/ KRAS/ EGFR/ RAP1/ SRC/ LYN/ ITGB1/ ITGA2/ ITGAV/ ITGB4/ EPHA2/ EPS8/ CTNNA"
non-EV: VDAC Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-producing cells
DLD-1
EV-harvesting Medium
Serum free medium
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Not specified
Pelleting: speed (g)
150000
Wash: time (min)
180
Wash: Rotor Type
Not specified
Wash: speed (g)
150000
Filtration steps
0.22µm or 0.2µm
Ultra filtration
Cut-off size (kDa)
100
Membrane type
Not specified
Characterization: Protein analysis
Protein Concentration Method
microBCA
Western Blot
Antibody details provided?
No
Lysis buffer provided?
Yes
Detected EV-associated proteins
"Flotillin1/ TSG101/ HSP70/ KRAS/ EGFR/ RAP1/ SRC/ LYN/ ITGB1/ ITGA2/ ITGAV/ ITGB4/ EPHA2/ EPS8/ CTNNA"
Not detected contaminants
VDAC
Characterization: Lipid analysis
No
Characterization: Particle analysis
NTA
Report type
Not Reported
EV concentration
Yes
Particle yield
"number of particles per microgram of protein" 1E9
|
||||||||
EV130036 | 1/1 | Homo sapiens | NAY | (d)(U)C | Colombo M | 2013 | 33% | |
Study summaryFull title
All authors
Colombo M, Moita C, van Niel G, Kowal J, Vigneron J, Benaroch P, Manel N, Moita LF, Théry C, Raposo G
Journal
J Cell Sci
Abstract
Exosomes are extracellular vesicles (EVs) secreted upon fusion of endosomal multivesicular bodies (M (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes / extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
209.7 (pelleting)
Protein markers
EV: CD81/ CD63/ MHC2/ Hsc70
non-EV: Proteomics
no
Show all info
Study aim
Biogenesis/Sorting
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Pelleting: rotor type
45Ti
Pelleting: adjusted k-factor
209.7
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD63/ CD81/ "Hsc70 / MHC2"
ELISA
Antibody details provided?
No
Detected EV-associated proteins
"Hsc70 / MHC2"
Flow cytometry specific beads
Antibody details provided?
No
Antibody dilution provided?
No
Selected surface protein(s)
Yes
Characterization: Particle analysis
EM
EM-type
immune EM
EM protein
CD63 ; MHC2
Image type
Close-up, Wide-field
|
||||||||
EV130086 | 2/5 | Homo sapiens | Pleural fluid |
(d)(U)C Filtration |
Chugh PE | 2013 | 33% | |
Study summaryFull title
All authors
Chugh PE, Sin SH, Ozgur S, Henry DH, Menezes P, Griffith J, Eron JJ, Damania B, Dittmer DP
Journal
PLoS Pathog
Abstract
MicroRNAs (miRNAs) are stable, small non-coding RNAs that modulate many downstream target genes. Rec (show more...)
EV-METRIC
33% (83rd percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Pleural fluid
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration Adj. k-factor
232.7 (pelleting) / 232.7 (washing)
Protein markers
EV: HSP90beta/ Beta-actin/ HSP90alpha/ Flotillin2
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Homo sapiens
Sample Type
Pleural fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Pelleting: rotor type
SW32
Pelleting: adjusted k-factor
232.7
Wash: volume per pellet (ml)
35
Wash: Rotor Type
SW32
Wash: adjusted k-factor
232.7
Filtration steps
0.22µm or 0.2µm
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
Flotillin2/ HSP90alpha/ HSP90beta/ Beta-actin
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Flotillin2/ HSP90alpha/ HSP90beta/ Beta-actin
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Close-up
|
||||||||
EV130066 | 2/2 | Bos bovis | Epididymal fluid | (d)(U)C | Caballero JN | 2013 | 33% | |
Study summaryFull title
All authors
Caballero JN, Frenette G, Belleannée C, Sullivan R
Journal
PLoS One
Abstract
Acquisition of fertilization ability by spermatozoa during epididymal transit occurs in part by the (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Epididymal fluid
Sample origin
NAY
Focus vesicles
microvesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: CD9
non-EV: Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Bos bovis
Sample Type
Epididymal fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 800 g and 10,000 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
70
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
CD9
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV130023 | 1/2 | Drosophila melanogaster | NAY |
(d)(U)C DG |
Beckett K | 2013 | 33% | |
Study summaryFull title
All authors
Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Le Borgne R, Vincent JP
Journal
Traffic
Abstract
Wingless acts as a morphogen in Drosophila wing discs, where it specifies cell fates and controls gr (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
DG Protein markers
EV: Flotillin2
non-EV: Proteomics
yes
EV density (g/ml)
1.17
Show all info
Study aim
Function
Sample
Species
Drosophila melanogaster
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
90
Density gradient
Only used for validation of main results
Yes
Lowest density fraction
0.25
Highest density fraction
2
Orientation
Bottom-up
Speed (g)
100000
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Flotillin2
ELISA
Antibody details provided?
No
Detected EV-associated proteins
Flotillin2
Characterization: Particle analysis
None
|
||||||||
EV130031 | 1/1 | Mus musculus | NAY | (d)(U)C | Basso M | 2013 | 33% | |
Study summaryFull title
All authors
Basso M, Pozzi S, Tortarolo M, Fiordaliso F, Bisighini C, Pasetto L, Spaltro G, Lidonnici D, Gensano F, Battaglia E, Bendotti C, Bonetto V
Journal
J Biol Chem
Abstract
Amyotrophic lateral sclerosis is the most common motor neuron disease and is still incurable. The me (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
exosomes
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV: Flotilin1/ VCP/p97/ SOD1
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Mus musculus
Sample Type
Cell culture supernatant
EV-harvesting Medium
serum free
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Characterization: Protein analysis
Western Blot
Antibody details provided?
Yes
Antibody dilution provided?
Yes
Detected EV-associated proteins
Flotilin1/ VCP/p97/ SOD1
ELISA
Antibody details provided?
No
Detected EV-associated proteins
VCP/p97/ SOD1
Characterization: Particle analysis
EM
EM-type
transmission EM/ immune EM
EM protein
SOD1
Image type
Close-up, Wide-field
|
||||||||
EV130029 | 1/1 | Homo sapiens | NAY | (d)(U)C | Aqil M | 2013 | 33% | |
Study summaryFull title
All authors
Aqil M, Naqvi AR, Bano AS, Jameel S
Journal
PLoS One
Abstract
The HIV-1 accessory protein Nef is an important virulence factor. It associates with cellular membra (show more...)
EV-METRIC
33% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Cell culture supernatant
Sample origin
NAY
Focus vesicles
Separation protocol
Separation protocol
(d)(U)C
Adj. k-factor
253.9 (pelleting) / 253.9 (washing)
Protein markers
EV: Alix/ CD81/ Beta-actin/ TSG101/ GAPDH
non-EV: Cell organelle protein Proteomics
no
Show all info
Study aim
Other/HIV-1 accessory protein function
Sample
Species
Homo sapiens
Sample Type
Cell culture supernatant
EV-harvesting Medium
EV Depleted
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 800 g and 10,000 g Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
60
Pelleting: rotor type
SW28
Pelleting: adjusted k-factor
253.9
Wash: volume per pellet (ml)
30
Wash: Rotor Type
SW28
Wash: adjusted k-factor
253.9
Characterization: Protein analysis
Western Blot
Antibody details provided?
No
Detected EV-associated proteins
Alix/ CD81/ TSG101/ GAPDH/ Beta-actin
Detected contaminants
Cell organelle protein
ELISA
Antibody details provided?
No
Detected EV-associated proteins
GAPDH/ Beta-actin
Characterization: Particle analysis
None
|
||||||||
EV130262 | 3/3 | Homo sapiens | Blood plasma |
DC (d)(U)C |
Haderk F | 2013 | 29% | |
Study summaryFull title
All authors
Haderk F, Hanna B, Richter K, Schnölzer M, Zenz T, Stilgenbauer S, Lichter P, Seiffert M.
Journal
Leuk Lymphoma
Abstract
Extracellular vesicles (EVs) are membrane-enclosed nanoparticles 30 to 1000 nm in size and represent (show more...)
EV-METRIC
29% (61st percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Blood plasma
Sample origin
chronic lymphocytic leukemia
Focus vesicles
extracellular vesicle
Separation protocol
Separation protocol
DC
(d)(U)C Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Identification of content (omics approaches)
Sample
Species
Homo sapiens
Sample Type
Blood plasma
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 10,000 g and 50,000 g Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Pelleting: rotor type
Not specified
Pelleting: speed (g)
100000
Wash: time (min)
120
Wash: Rotor Type
Not specified
Wash: speed (g)
100000
Density cushion
Density medium
Sucrose
Characterization: Protein analysis
Protein Concentration Method
microBCA
Proteomics database
No
Characterization: Lipid analysis
No
EM
EM-type
Transmission-EM
Image type
Close-up, Wide-field
Report size (nm)
|
||||||||
EV130134 | 1/1 | Gram-negative bacteria | Indoor dust |
(d)(U)C Filtration UF |
Kim YS | 2013 | 29% | |
Study summaryFull title
All authors
Kim YS, Choi EJ, Lee WH, Choi SJ, Roh TY, Park J, Jee YK, Zhu Z, Koh YY, Gho YS, Kim YK
Journal
Clin Exp Allergy
Abstract
BACKGROUND: Many bacterial components in indoor dust can evoke inflammatory pulmonary diseases. Bact (show more...)
EV-METRIC
29% (70th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Indoor dust
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Filtration UF Adj. k-factor
139.8 (pelleting)
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Function
Sample
Species
Gram-negative bacteria
Sample Type
Indoor dust
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
180
Pelleting: rotor type
45Ti
Pelleting: adjusted k-factor
139.8
Filtration steps
0.45µm > x > 0.22µm, 0.22µm or 0.2µm
Characterization: Particle analysis
DLS
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV130076 | 2/3 | Gut microbiota | Mouse intestinal fluid |
(d)(U)C DG Filtration |
Kang CS | 2013 | 29% | |
Study summaryFull title
All authors
Kang CS, Ban M, Choi EJ, Moon HG, Jeon JS, Kim DK, Park SK, Jeon SG, Roh TY, Myung SJ, Gho YS, Kim JG, Kim YK
Journal
PLoS One
Abstract
Gut microbiota play an important part in the pathogenesis of mucosal inflammation, such as inflammat (show more...)
EV-METRIC
29% (50th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
Mouse intestinal fluid
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
DG Filtration Protein markers
EV:
non-EV: Proteomics
yes
Show all info
Study aim
Function
Sample
Species
Gut microbiota
Sample Type
Mouse intestinal fluid
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Between 10,000 g and 50,000 g
Between 100,000 g and 150,000 g Equal to or above 150,000 g Pelleting performed
Yes
Pelleting: time(min)
120
Density gradient
Only used for validation of main results
Yes
Speed (g)
100000
Filtration steps
0.45µm > x > 0.22µm,
Characterization: Protein analysis
Characterization: Particle analysis
EM
EM-type
transmission EM
Image type
Wide-field
|
||||||||
EV130139 | 1/3 | Homo sapiens | "Cerebrospinal fluid" | (d)(U)C | Carone C | 2013 | 29% | |
Study summaryFull title
All authors
Carone C, Genedani S, Leo G, Filaferro M, Fuxe K, Agnati LF
Journal
Mol Ther Nucleic Acids
Abstract
Development of biofluid-based molecular diagnostic tests for cancer is an important step towards tum (show more...)
EV-METRIC
29% (75th percentile of all experiments on the same sample type)
Reported
Not reported Not applicable EV-enriched
proteins
Protein analysis: analysis of three or more EV-enriched proteins
non
EV-enriched protein
Protein analysis: assessment of a non-EV-enriched protein
qualitative
and quantitative analysis
Particle analysis: implementation of both qualitative and quantitative methods. For the quantitative method, the reporting of measured EV concentration is expected.
electron
microscopy images
Particle analysis: inclusion of a widefield and close-up electron microscopy image
density
gradient
Separation method: density gradient, at least as validation of results attributed to EVs
EV density
Separation method: reporting of obtained EV density
ultracentrifugation specifics
Separation method: reporting of g-forces, duration and rotor type of ultracentrifugation steps
antibody
specifics
Protein analysis: antibody clone/reference number and dilution
lysate
preparation
Protein analysis: lysis buffer composition
Study dataSample type
"Cerebrospinal fluid"
Sample origin
NAY
Focus vesicles
extracellular vesicles
Separation protocol
Separation protocol
(d)(U)C
Protein markers
EV:
non-EV: Proteomics
no
Show all info
Study aim
Omics
Sample
Species
Homo sapiens
Sample Type
"Cerebrospinal fluid"
Separation Method
(Differential) (ultra)centrifugation
dUC: centrifugation steps
Below or equal to 800 g
Between 100,000 g and 150,000 g Pelleting performed
Yes
Pelleting: time(min)
80
Characterization: Particle analysis
NTA
EM
EM-type
transmission EM/ cryo EM
Image type
Close-up, Wide-field
|
||||||||
51 - 100 of 384 | keyboard_arrow_leftkeyboard_arrow_right |